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BitBoo shows Wario, the popular video game antagonist, saying "Boo!" vehemently. It is used to show that the viewer is feeling outraged, offended, or disgusted. It is the counterpart of the now deceased emote bitWah, which featured fellow antagonist Waluigi.
BitBoo was introduced on April 28, 2019, following 88bitmusic attaining 1400 subs, and was created by Ckibe.
BitBoo was featured in Trevor's Thoughts, where it appeared whenever a viewer expressed disagreement with Trevor Gomes.